Anti-MRSA activity of a bioactive compound produced by a marine Streptomyces and its optimization using statistical experimental design.

ABSTRACT:

Objectives

To address the alarming problem of methicillin-resistant Staphylococcus aureus (MRSA), herein, a marine Streptomyces capable of producing an anti-MRSA compound has been studied.

Materials and methods

Strain MN41 was morphologically and physiologically characterized and then, molecularly identified using 16SrRNA analysis. To produce the bioactive compound in large scale, a kind of submerged liquid fermentation was adopted. The antibacterial agent was purified using a silica gel column followed by a semi-preparative HPLC and the isolated metabolite was identified using mass spectrometry, Nuclear magnetic resonance (NMR) and Fourier-transform infrared (FTIR). Finally, the production process was subjected to a two steps optimization using Plackett-Burman

SUBMITTER: Norouzi H 

PROVIDER: S-EPMC6880529 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature