Effect of calcium on the interaction of Acinetobacter baumannii with human respiratory epithelial cells.

ABSTRACT:

Background

Investigating the factors that influence Acinetobacter baumannii(Ab) adhesion/invasion of host cells is important to understand its pathogenicity. Metal cations have been shown to play an important role in regulating the biofilm formation and increasing the virulence of Ab; however, the effect of calcium on host-bacterial interaction has yet to be clarified. Here, the dynamic process of the interaction between Ab and human respiratory epithelial cells and the effect of calcium on host-bacterial interaction were explored using microscopic imaging, quantitative PCR and real time cellular analysis (RTCA).

Results

The concentration of calcium, multiplicity of infection and co-culture time were all demonstrated to have effects on host-bacterial interaction. A unique "d

SUBMITTER: Chen Y 

PROVIDER: S-EPMC6880639 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature