Microbiota-driven gut vascular barrier disruption is a prerequisite for non-alcoholic steatohepatitis development.

ABSTRACT:

Background & aims

Fatty liver disease, including non-alcoholic fatty liver (NAFLD) and steatohepatitis (NASH), has been associated with increased intestinal barrier permeability and translocation of bacteria or bacterial products into the blood circulation. In this study, we aimed to unravel the role of both intestinal barrier integrity and microbiota in NAFLD/NASH development.

Methods

C57BL/6J mice were fed with high-fat diet (HFD) or methionine-choline-deficient diet for 1 week or longer to recapitulate aspects of NASH (steatosis, inflammation, insulin resistance). Genetic and pharmacological strategies were then used to modulate intestinal barrier integrity.

Results

We show that disruption of the intestinal epithelial barrier and gut vascular barrier (GVB) are earl

SUBMITTER: Mouries J 

PROVIDER: S-EPMC6880766 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature