Dysregulated miRNome of plasmacytoid dendritic cells from patients with Sjogren's syndrome is associated with processes at the centre of their function.

ABSTRACT:

Objective

A considerable body of evidence supports a role for type-I IFN in the pathogenesis of primary SS (pSS). As plasmacytoid dendritic cells (pDCs) are a major source of type-I IFN, we investigated their molecular regulation by measuring expression of a large set of miRNAs.

Methods

pDCs were isolated from peripheral blood of pSS patients (n = 30) and healthy controls (n = 16) divided into two independent cohorts (discovery and replication). Screening of 758 miRNAs was assessed by an OpenArray quantitative PCR-based technique; replication of a set of identified miRNAs was performed by custom array. Functional annotation of miRNA targets was performed using pathway enrichment. Novel targets of miR-29a and miR-29c were identified using a proteomic approach (stable isotope

SUBMITTER: Hillen MR 

PROVIDER: S-EPMC6880856 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature