Maintenance of hPSCs under Xeno-Free and Chemically Defined Culture Conditions.

ABSTRACT: Previously, the majority of human embryonic stem cells and human induced pluripotent stem cells have been derived on feeder layers and chemically undefined medium. Those media components related to feeder cells, or animal products, often greatly affect the consistency of the cell culture. There are clear advantages of a defined, xeno-free, and feeder-free culture system for human pluripotent stem cells (hPSCs) cultures, since consistency in the formulations prevents lot-to-lot variability. Eliminating all non-human components reduces health risks for downstream applications, and those environments reduce potential immunological reactions from stem cells. Therefore, development of feeder-free hPSCs culture systems has been an important focus of hPSCs research. Recently, researchers have est

SUBMITTER: Lim JJ 

PROVIDER: S-EPMC6881038 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature