Improvement of Human Sperm Vacuolization and DNA Fragmentation Co-Cultured with Adipose-Derived Mesenchymal Stem Cell Secretome: In Vitro Effect.

ABSTRACT:

Background and objectives

Oxidative stress (OS) is known to be an important factor of male infertility. Adipose-derived mesenchymal stem cells (AD-MSCs) are known to have immune-modulatory and anti-oxidant effects through their secretions, hence raising the idea of their potential benefit to improve sperm parameters. This study aims at investigating the effect of AD-MSCs conditioned medium (CM) on human sperm parameters in the presence and absence of H₂O₂-induced OS.

Methods and results

Sperm samples were collected from 30 healthy men and divided into two groups: non-stressed and H₂O₂-stressed. Isolated AD-MSCs from healthy donors undergoing liposuction were cultured and CM was collected at 24, 48 and 72 h. Both sperm groups were c

SUBMITTER: Bader R 

PROVIDER: S-EPMC6881046 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature