In vivo-mimicking microfluidic perfusion culture of pancreatic islet spheroids.

ABSTRACT: Native pancreatic islets interact with neighboring cells by establishing three-dimensional (3D) structures, and are surrounded by perfusion at an interstitial flow level. However, flow effects are generally ignored in islet culture models, although cell perfusion is known to improve the cell microenvironment and to mimic in vivo physiology better than static culture systems. Here, we have developed functional islet spheroids using a microfluidic chip that mimics interstitial flow conditions with reduced shear cell damage. Dynamic culture, compared to static culture, enhanced islet health and maintenance of islet endothelial cells, reconstituting the main component of islet extracellular matrix within spheroids. Optimized flow condition allowed localization of secreted soluble factors near

SUBMITTER: Jun Y 

PROVIDER: S-EPMC6881167 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature