Strain improvement of Escherichia coli K-12 for recombinant production of deuterated proteins.

ABSTRACT: Deuterium isotope labelling is important for structural biology methods such as neutron protein crystallography, nuclear magnetic resonance and small angle neutron scattering studies of proteins. Deuterium is a natural low abundance stable hydrogen isotope that in high concentrations negatively affect growth of cells. The generation time for Escherichia coli K-12 in deuterated medium is substantially increased compared to cells grown in hydrogenated (protiated) medium. By using a mutagenesis plasmid based approach we have isolated an E. coli strain derived from E. coli K-12 substrain MG1655 that show increased fitness in deuterium based growth media, without general adaptation to media components. By whole-genome sequencing we identified the genomic changes in the obtained strain and show

SUBMITTER: Kelpsas V 

PROVIDER: S-EPMC6881287 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature