Quantification of phosphoinositides reveals strong enrichment of PIP₂ in HIV-1 compared to producer cell membranes.

ABSTRACT: Human immunodeficiency virus type 1 (HIV-1) acquires its lipid envelope during budding from the plasma membrane of the host cell. Various studies indicated that HIV-1 membranes differ from producer cell plasma membranes, suggesting budding from specialized membrane microdomains. The phosphoinositide PI(4,5)P₂ has been of particular interest since PI(4,5)P₂ is needed to recruit the viral structural polyprotein Gag to the plasma membrane and thus facilitates viral morphogenesis. While there is evidence for an enrichment of PIP₂ in HIV-1, fully quantitative analysis of all phosphoinositides remains technically challenging and therefore has not been reported, yet. Here, we present a comprehensive analysis of the lipid content of HIV-1 and of plasma membranes fr

SUBMITTER: Mucksch F 

PROVIDER: S-EPMC6881329 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature