Glycerol not lactate is the major net carbon source for gluconeogenesis in mice during both short and prolonged fasting.

ABSTRACT:

Objective

Fasting results in major metabolic changes including a switch from glycogenolysis to gluconeogenesis to maintain glucose homeostasis. However, the relationship between the length of fasting and the relative contribution of gluconeogenic substrates remains unclear. We investigated the relative contribution of glycogen, lactate, and glycerol in glucose production of male C57BL/6 J-albino mice after 6, 12, and 18 h of fasting.

Methods

We used non-perturbative infusions of ¹³C₃ lactate, ¹³C₃ glycerol, and ¹³C₆ glucose combined with liquid chromatography mass spectrometry and metabolic flux analysis to study the contribution of substrates in gluconeogenesis (GNG).

Results

During infusion studies, b

SUBMITTER: Wang Y 

PROVIDER: S-EPMC6881678 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature