Metagenomic Signatures of Gut Infections Caused by Different Escherichia coli Pathotypes.

ABSTRACT: Escherichia coli is a leading contributor to infectious diarrhea and child mortality worldwide, but it remains unknown how alterations in the gut microbiome vary for distinct E. coli pathotype infections and whether these signatures can be used for diagnostic purposes. Further, the majority of enteric diarrheal infections are not diagnosed with respect to their etiological agent(s) due to technical challenges. To address these issues, we devised a novel approach that combined traditional, isolate-based and molecular-biology techniques with metagenomics analysis of stool samples and epidemiological data. Application of this pipeline to children enrolled in a case-control study of diarrhea in Ecuador showed that, in about half of the cases where an E. coli pathotype was

SUBMITTER: Pena-Gonzalez A 

PROVIDER: S-EPMC6881795 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature