Comparison of visceral adipose tissue DNA methylation and gene expression profiles in female adolescents with obesity.

ABSTRACT:

Background

Epigenetic changes in visceral adipose tissue (VAT) with obesity and their effects on gene expression are poorly understood, especially during emergent obesity in youth. The current study tested the hypothesis that methylation and gene expression profiles of key growth factor and inflammatory pathways are altered in VAT from obese compared to non-obese youth.

Methods

VAT samples from adolescent females grouped as Lean (L; n = 15; age = 15 ± 3 years, BMI = 21.9 ± 3.0 kg/m²) or Obese (Ob; n = 15, age = 16 ± 2 years, BMI = 45.8 ± 9.8 kg/m²) were collected. Global methylation (n = 20) and gene expression (N = 30) patterns were profiled via microarray and interrogated for differences between groups by ANCOVA (p < 0.05), followed by biological path

SUBMITTER: Barberio MD 

PROVIDER: S-EPMC6881970 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature