CTLA-4 Immunohistochemistry and Quantitative Image Analysis for Profiling of Human Cancers.

ABSTRACT: There is an important need in immuno-oncology to develop reliable immunohistochemistry (IHC) to assess the expression of CTLA-4⁺ tumor-infiltrating lymphocytes in human cancers and quantify them with image analysis (IA). We used commercial polyclonal and monoclonal antibodies and characterized three chromogenic cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) assays with suitable specificity and sensitivity for use in formalin-fixed, paraffin-embedded (FFPE) tissues. We found variable numbers of CTLA-4⁺ lymphocytes in multiple types of cancer and secondary lymphoid organs (SLOs) and other normal human tissues. Combining CTLA-4 with CD3, CD4, or CD8 by immunofluorescence showed that CTLA-4⁺ lymphocytes in SLOs and tumors were typically CD3⁺ an

SUBMITTER: Brown C 

PROVIDER: S-EPMC6882065 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature