An efficient system composed of maize protoplast transfection and HPLC-MS for studying the biosynthesis and regulation of maize benzoxazinoids.

ABSTRACT:

Background

Insect herbivory poses a major threat to maize. Benzoxazinoids are important anti-insect secondary metabolites in maize, whose biosynthetic pathway has been extensively studied. However, yet little is known about how benzoxazinoids are regulated in maize, partly due to lack of mutant resources and recalcitrance to genetic transformation. Transient systems based on mesophyll- or cultured cell-derived protoplasts have been exploited in several plant species and have become a powerful tool for rapid or high-throughput assays of gene functions. Nevertheless, these systems have not been exploited to study the regulation of secondary metabolites.

Results

A protocol for isolation of protoplasts from etiolated maize seedlings and efficient transfection was optimized. Furt

SUBMITTER: Gao L 

PROVIDER: S-EPMC6882228 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature