Engineering integrative vectors based on phage site-specific recombination mechanism for Lactococcus lactis.

ABSTRACT:

Background

Site-specific integration system allows foreign DNA to be integrated into the specific site of the host genome, enabling stable expression of heterologous protein. In this study, integrative vectors for secretion and surface display of proteins were constructed based on a lactococcal phage TP901-1 integrating system.

Results

The constructed integration system comprises of a lactococcal promoter (P_nisA or P₁₇₀), phage attachment site (attP) from bacteriophage TP901-1, a signal peptide (USP45 or SPK1) for translocation of the target protein, and a PrtP₃₄₄ anchor domain in the case of the integrative vectors for surface display. There were eight successfully constructed integrative vectors with each having a different combination of p

SUBMITTER: Koko I 

PROVIDER: S-EPMC6882331 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature