RNA-binding protein KHSRP promotes tumor growth and metastasis in non-small cell lung cancer.

ABSTRACT:

Background

KH-type splicing regulatory protein (KHSRP) plays an important role in cancer invasion, but the relevant mechanism is not well known. In the present study, we investigated the function and potential molecular mechanism of KHSRP in non-small cell lung cancer (NSCLC) metastasis and elucidated its clinical significance.

Methods

Isobaric tags for relative and absolute quantitation and the SWATH™ approach were combined with nanoliquid chromatography-tandem mass spectrometry analysis to identify metastasis-associated nucleoproteins in NSCLC. Real-time PCR and Western blot were used to screen for metastasis-associated candidate molecules. Gene knockdown and overexpression were used to investigate their functions and molecular mechanisms in lung cancer cells. Coimmunoprec

SUBMITTER: Yan M 

PROVIDER: S-EPMC6882349 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature