"A Step and a Ceiling": mechanical properties of Ca²⁺ spark vasoregulation in resistance arteries by pressure-induced oxidative activation of PKG.

ABSTRACT: We investigated the biomechanical relationship between intraluminal pressure within small mesenteric resistance arteries, oxidant activation of PKG, Ca²⁺ sparks, and BK channel vasoregulation. Mesenteric resistance arteries from wild type (WT) and genetically modified mice with PKG resistance to oxidative activation were studied using wire and pressure myography. Ca²⁺ sparks and Ca²⁺ transients within vascular smooth muscle cells of intact arteries were characterized using high-speed confocal microscopy of intact arteries. Arteries were studied under conditions of varying intraluminal pressure and oxidation. Intraluminal pressure specifically, rather than the generic stretch of the artery, was necessary to activate the oxidative pathway. We demonstrated a g

SUBMITTER: Csato V 

PROVIDER: S-EPMC6883097 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature