Testis-specific Arf promoter expression in a transposase-aided BAC transgenic mouse model.

ABSTRACT: CDKN2A is an evolutionarily conserved gene encoding proteins implicated in tumor suppression, ocular development, aging, and metabolic diseases. Like the human form, mouse Cdkn2a encodes two distinct proteins-p16^Ink4a, which blocks cyclin-dependent kinase activity, and p19^Arf, which is best known as a positive regulator of the p53 tumor suppressor-and their functions have been well-studied in genetically engineered mouse models. Relatively little is known about how expression of the two transcripts is controlled in normal development and in certain disease states. To better understand their coordinate and transcript-specific expression in situ, we used a transposase-aided approach to generate a new BAC transgenic mouse model in which the first exons encoding Arf and I

SUBMITTER: Sung CY 

PROVIDER: S-EPMC6883165 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature