Comparative analysis of the complete chloroplast genome sequences of six species of Pulsatilla Miller, Ranunculaceae.

ABSTRACT:

Background

Baitouweng is a traditional Chinese medicine with a long history of different applications. Although referred to as a single medicine, Baitouweng is actually comprised of many closely related species. It is therefore critically important to identify the different species that are utilized in these medicinal applications. Knowledge about their phylogenetic relationships can be derived from their chloroplast genomes and may provide additional insights into development of molecular markers.

Methods

Genomic DNA was extracted from six species of Pulsatilla and then sequenced on an Illumina HiSeq 4000. Sequences were assembled into contigs by SOAPdenovo 2.04, aligned to the reference genome using BLAST, and then manually corrected. Genome annotation was performed

SUBMITTER: Zhang T 

PROVIDER: S-EPMC6883693 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature