Fecal Coliform Bacterial Detection to Assess Enema Adherence in HIV Prevention Clinical Studies.

ABSTRACT: Evaluating the efficacy of any HIV prevention strategy is dependent on ensuring and objectively monitoring adherence to the intervention. Medicated rectal enemas are a potential method for providing topical, episodic HIV prophylaxis during receptive anal intercourse. Assessing adherence to recommended enema dosing regimens is essential in evaluating the utility of this strategy. We utilized fecal coliform bacteria on used enema tips as a marker for enema use. Enema tip coliforms were tested by repurposing a microtiter plate-based water quality test designed to detect fecal contamination of water. Coliform detection occurred with 100% sensitivity and specificity when tips were assayed on day of use. The assay performed well post-7 day sample storage at room temperature, yielding a sensitivi

SUBMITTER: Aung WS 

PROVIDER: S-EPMC6883768 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature