Single-cell transcriptomics of the human retinal pigment epithelium and choroid in health and macular degeneration.

ABSTRACT: The human retinal pigment epithelium (RPE) and choroid are complex tissues that provide crucial support to the retina. Disease affecting either of these supportive tissues can lead to irreversible blindness in the setting of age-related macular degeneration. In this study, single-cell RNA sequencing was performed on macular and peripheral regions of RPE-choroid from 7 human donor eyes in 2 independent experiments. In the first experiment, total RPE/choroid preparations were evaluated and expression profiles specific to RPE and major choroidal cell populations were identified. As choroidal endothelial cells represent a minority of the total RPE/choroidal cell population but are strongly implicated in age-related macular degeneration (AMD) pathogenesis, a second single-cell RNA-sequencing ex

SUBMITTER: Voigt AP 

PROVIDER: S-EPMC6883845 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature