Prenatal electroporation-mediated gene transfer restores Slc26a4 knock-out mouse hearing and vestibular function.

ABSTRACT: The otocyst, an anlage of the inner ear, presents an attractive target to study treatment strategies for genetic hearing loss and inner ear development. We have previously reported that electroporation-mediated transuterine gene transfer of Connexin30, utilizing a monophasic pulse into Connexin30^-/- mouse otocysts at embryonic day 11.5, is able to prevent putative hearing deterioration. However, it is not clear whether supplementary gene transfer can rescue significant morphological changes, caused by genetic deficits. In addition, with the transuterine gene transfer technique utilized in our previous report, the survival rate of embryos and their mothers after treatment was low, which became a serious obstacle for effective in vivo experiments. Here, we set out to elucidate the

SUBMITTER: Takeda H 

PROVIDER: S-EPMC6884448 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature