Identification of Acinetobacter baumannii and its carbapenem-resistant gene bla_OXA-23-like by multiple cross displacement amplification combined with lateral flow biosensor.

ABSTRACT: Acinetobacter baumannii is a frequent cause of the nosocomial infections. Herein, a novel isothermal amplification technique, multiple cross displacement amplification (MCDA) is employed for detecting all A. baumannii strains and identifying the strains harboring bla_OXA-23-like gene. The duplex MCDA assay, which targets the pgaD and bla_OXA-23-like genes, could identify the A. baumannii isolates and differentiate these isolates harboring bla_OXA-23-like gene. The disposable lateral flow biosensors (LFB) were used for analyzing the MCDA products. A total of sixty-eight isolates, include fifty-three A. baumannii strains and fifteen non-A. baumannii strains, were employed to optimize MCDA methods and determine the sensitivity, specificity and feasibility. The op

SUBMITTER: Hu S 

PROVIDER: S-EPMC6884502 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature