DART-seq: an antibody-free method for global m⁶A detection.

ABSTRACT: N⁶-methyladenosine (m⁶A) is a widespread RNA modification that influences nearly every aspect of the messenger RNA lifecycle. Our understanding of m⁶A has been facilitated by the development of global m⁶A mapping methods, which use antibodies to immunoprecipitate methylated RNA. However, these methods have several limitations, including high input RNA requirements and cross-reactivity to other RNA modifications. Here, we present DART-seq (deamination adjacent to RNA modification targets), an antibody-free method for detecting m⁶A sites. In DART-seq, the cytidine deaminase APOBEC1 is fused to the m⁶A-binding YTH domain. APOBEC1-YTH expression in cells induces C-to-U deamination at sites adjacent to m⁶A residues, wh

SUBMITTER: Meyer KD 

PROVIDER: S-EPMC6884681 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature