PD1Hi CD8+ T cells correlate with exhausted signature and poor clinical outcome in hepatocellular carcinoma.

ABSTRACT: BACKGROUND:CD8+ T cells differentiate into exhausted status within tumors, including hepatocellular carcinoma (HCC), which constitutes a solid barrier to effective anti-tumor immunity. A detailed characterization of exhausted T cells and their prognostic value in HCC is lacking. METHODS:We collected fresh tumor tissues with adjacent non-tumor liver tissues and blood specimens of 56 HCC patients, as well as archived samples from two independent cohorts of HCC patients (n = 358 and n = 254), who underwent surgical resection. Flow cytometry and multiplex immunostaining were used to characterize CD8+ T cells. Patient prognosis was evaluated by Kaplan-Meier analysis and Cox regression analysis. RESULTS:CD8+ T cells were classified into three distinct subpopulations: PD1Hi, PD1Int and PD1-. PD1H

SUBMITTER: Ma J 

PROVIDER: S-EPMC6884778 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature