Development of clinically relevant in vivo metastasis models using human bone discs and breast cancer patient-derived xenografts.

ABSTRACT:

Background

Late-stage breast cancer preferentially metastasises to bone; despite advances in targeted therapies, this condition remains incurable. The lack of clinically relevant models for studying breast cancer metastasis to a human bone microenvironment has stunted the development of effective treatments for this condition. To address this problem, we have developed humanised mouse models in which breast cancer patient-derived xenografts (PDXs) metastasise to human bone implants with low variability and high frequency.

Methods

To model the human bone environment, bone discs from femoral heads of patients undergoing hip replacement surgery were implanted subcutaneously into NOD/SCID mice. For metastasis studies, 7 patient-derived xenograft tumours (PDX: BB3RC32, ER+ PR+ HE

SUBMITTER: Lefley D 

PROVIDER: S-EPMC6884811 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature