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Proteomic analysis of Nrk gene-disrupted placental tissue cells explains physiological significance of NRK.

ABSTRACT: OBJECTIVE:NRK is a unique X chromosome-linked protein kinase expressed predominantly in placenta. The gene knockout causes placental overgrowth and delayed labor of Nrk-null fetuses from dams in mouse. To clarify unknown mechanisms behind the Nrk-null phenotypes, protein expression profiles were analyzed in the Nrk-null placenta using a high-performance two-dimensional electrophoresis methodology. RESULTS:Among around 1800 spots detected, we characterized a dozen protein spots whose expression levels were significantly altered in the Nrk-null placenta compared to wild-type. Analyzing these data sets is expected to reflect the difference physiologically in the presence or absence of NRK, facilitating the development of therapeutic strategies.

SUBMITTER: Denda K

PROVIDER: S-EPMC6884884 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Proteomic analysis of Nrk gene-disrupted placental tissue cells explains physiological significance of NRK.

Denda Kimitoshi K Ida Kanako K Tanno Masataka M Nakao-Wakabayashi Kanako K Komada Masayuki M Hayashi Nobuhiro N

BMC research notes 20191129 1

<h4>Objective</h4>NRK is a unique X chromosome-linked protein kinase expressed predominantly in placenta. The gene knockout causes placental overgrowth and delayed labor of Nrk-null fetuses from dams in mouse. To clarify unknown mechanisms behind the Nrk-null phenotypes, protein expression profiles were analyzed in the Nrk-null placenta using a high-performance two-dimensional electrophoresis methodology.<h4>Results</h4>Among around 1800 spots detected, we characterized a dozen protein spots who ...[more]

PMID: 31783915

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