Fhl1p protein, a positive transcription factor in Pichia pastoris, enhances the expression of recombinant proteins.

ABSTRACT:

Background

The methylotrophic yeast Pichia pastoris is well-known for the production of a broad spectrum of functional types of heterologous proteins including enzymes, antigens, engineered antibody fragments, and next gen protein scaffolds and many transcription factors are utilized to address the burden caused by the high expression of heterologous proteins. In this article, a novel P. pastoris transcription factor currently annotated as Fhl1p, an activator of ribosome biosynthesis processing, was investigated for promoting the expression of the recombinant proteins.

Results

The function of Fhl1p of P. pastoris for improving the expression of recombinant proteins was verified in strains expressing phytase, pectinase and mRFP, showing that the productivity was increased by

SUBMITTER: Zheng X 

PROVIDER: S-EPMC6884909 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature