Inhibition of ISG15 Enhances the Anti-Cancer Effect of Trametinib in Colon Cancer Cells.

ABSTRACT:

Background

Colon cancer is one of the most common cancers worldwide. IFN-stimulated gene 15 (ISG15), a ubiquitin-like molecule, is strongly up-regulated by type I interferon as a crucial response to a variety of microbial and cellular stress stimuli. However, the role of ISG15 in colon cancer remains unclear.

Methods

The expression of ISG15 in colon cancer tissues and cell lines was detected by using Western blotting and immunohistochemistry. ISG15 expression levels of colon cancer cells treated with trametinib was verified by using the data downloaded from the Gene Expression Omnibus (GEO) databases, quantitative real-time PCR analysis and Western blots assays. ISG15-siRNA was used to silence ISG15 in colon cancer cell line to determine the roles of ISG15 in colon cancer ce

SUBMITTER: Zhou S 

PROVIDER: S-EPMC6884973 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature