PRIMA-1^MET Induces Cellular Senescence and Apoptotic Cell Death in Cholangiocarcinoma Cells.

ABSTRACT:

Background/aim

This study examined the in vitro effects of the bile duct cancer drug PRIMA-1^MET on cholangiocarcinoma (CCA) cell growth to determine its potential usefulness in CCA therapy.

Materials and methods

The effect of this drug on the expression of senescent markers (p16^INK4A and p21) and the phosphorylation of p53 was investigated, as was the association between senescent markers and the patients' clinicopathological data.

Results

PRIMA-1^MET inhibited CCA cell growth with the half maximal-inhibitory concentration (IC₅₀) values of 21.9-40.8 μM. PRIMA-1^MET induced phospho-p53, p16^INK4A and p21 triggering cellular senescence and apoptosis. High expressions of p16^INK4A and p21 were assoc

SUBMITTER: Piyawajanusorn C 

PROVIDER: S-EPMC6885363 | biostudies-literature | 2019 Nov-Dec

REPOSITORIES: biostudies-literature