Glucose and glutamine metabolism in relation to mutational status in NSCLC histological subtypes.

ABSTRACT:

Background

Both hypoxia and oncogenic mutations rewire tumor metabolism. In this study, glucose and glutamine metabolism-related markers were examined in stage I - resectable stage IIIA non-small cell lung cancer (NSCLC). Furthermore, expression of metabolism-related markers was correlated with mutational status to examine mutations associated with rewired tumor metabolism.

Methods

Mutation analysis was performed for 97 tumors. Glucose and glutamine metabolism-related marker expression was measured by immunofluorescent staining (protein) and qPCR (mRNA) (n = 81).

Results

Glutamine metabolism-related markers were significantly higher in adeno- than squamous cell NSCLCs. Glucose transporter 1 (GLUT1) protein expression was higher in solid compared to lepidic adenocarcin

SUBMITTER: Meijer TWH 

PROVIDER: S-EPMC6885430 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature