The transcription factor ACE3 controls cellulase activities and lactose metabolism via two additional regulators in the fungus Trichoderma reesei.

ABSTRACT: Fungi of the genus Trichoderma are a rich source of enzymes, such as cellulases and hemicellulases, that can degrade lignocellulosic biomass and are therefore of interest for biotechnological approaches seeking to optimize biofuel production. The essential transcription factor ACE3 is involved in cellulase production in Trichoderma reesei; however, the mechanism by which ACE3 regulates cellulase activities is unknown. Here, we discovered that the nominal ace3 sequence in the T. reesei genome available through the Joint Genome Institute is erroneously annotated. Moreover, we identified the complete ace3 sequence, the ACE3 Zn(II)₂Cys₆ domain, and the ACE3 DNA-binding sites containing a 5'-CGGAN(T/A)₃-3' consensus. We found t

SUBMITTER: Zhang J 

PROVIDER: S-EPMC6885621 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature