Glycoengineering of chimeric antigen receptor (CAR) T-cells to enforce E-selectin binding.

ABSTRACT: Tissue colonization (homing) by blood-borne cells critically hinges on the ability of the cells to adhere to vascular endothelium with sufficient strength to overcome prevailing hemodynamic shear stress. These adhesive interactions are most effectively engendered via binding of the endothelial lectin E-selectin (CD62E) to its cognate ligand, sialyl Lewis-X (sLe ^X ), displayed on circulating cells. Although chimeric antigen receptor (CAR) T-cell immunotherapy holds promise for treatment of various hematologic and non-hematologic malignancies, there is essentially no information regarding the efficiency of CAR T-cell homing. Accordingly, we performed integrated biochemical studies and adhesion assays to examine the capacity of human CAR T-cells to engage E-selectin. Our dat

SUBMITTER: Mondal N 

PROVIDER: S-EPMC6885642 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature