Translational offsetting as a mode of estrogen receptor α-dependent regulation of gene expression.

ABSTRACT: Estrogen receptor alpha (ERα) activity is associated with increased cancer cell proliferation. Studies aiming to understand the impact of ERα on cancer-associated phenotypes have largely been limited to its transcriptional activity. Herein, we demonstrate that ERα coordinates its transcriptional output with selective modulation of mRNA translation. Importantly, translational perturbations caused by depletion of ERα largely manifest as "translational offsetting" of the transcriptome, whereby amounts of translated mRNAs and corresponding protein levels are maintained constant despite changes in mRNA abundance. Transcripts whose levels, but not polysome association, are reduced following ERα depletion lack features which limit translation efficiency including structured 5'UTRs and miRNA targe

SUBMITTER: Lorent J 

PROVIDER: S-EPMC6885737 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature