Cul3-Klhl18 ubiquitin ligase modulates rod transducin translocation during light-dark adaptation.

ABSTRACT: Adaptation is a general feature of sensory systems. In rod photoreceptors, light-dependent transducin translocation and Ca²⁺ homeostasis are involved in light/dark adaptation and prevention of cell damage by light. However, the underlying regulatory mechanisms remain unclear. Here, we identify mammalian Cul3-Klhl18 ubiquitin ligase as a transducin translocation modulator during light/dark adaptation. Under dark conditions, Klhl18^-/- mice exhibited decreased rod light responses and subcellular localization of the transducin α-subunit (Tα), similar to that observed in light-adapted Klhl18^+/+ mice. Cul3-Klhl18 promoted ubiquitination and degradation of Unc119, a rod Tα-interacting protein. Unc119 overexpression phenocopied Tα mislocalization observed in Klhl18

SUBMITTER: Chaya T 

PROVIDER: S-EPMC6885741 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature