Nck adapter proteins promote podosome biogenesis facilitating extracellular matrix degradation and cancer invasion.

ABSTRACT:

Background

Podosomes are membrane-bound adhesive structures formed by actin remodeling. They are capable of extracellular matrix (ECM) degradation, which is a prerequisite for cancer cell invasion and metastasis. The signaling mechanism of podosome formation is still unknown in cancer. We previously reported that Nck adaptors regulate directional cell migration and endothelial lumen formation by actin remodeling, while deficiency of Nck reduces cancer metastasis. This study evaluated the role of Nck adaptors in podosome biogenesis and cancer invasion.

Methods

This study was conducted in vitro using both healthy cells (Human Umbilical Vein Endothelial Cell, 3T3 fibroblasts) and cancer cells (prostate cancer cell line; PC3, breast cancer cell line; MDA-MB-231). Confocal and TI

SUBMITTER: Chaki SP 

PROVIDER: S-EPMC6885876 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature