Statistical modeling of STR capillary electrophoresis signal.

ABSTRACT:

Background

In order to isolate an individual's genotype from a sample of biological material, most laboratories use PCR and Capillary Electrophoresis (CE) to construct a genetic profile based on polymorphic loci known as Short Tandem Repeats (STRs). The resulting profile consists of CE signal which contains information about the length and number of STR units amplified. For samples collected from the environment, interpretation of the signal can be challenging given that information regarding the quality and quantity of the DNA is often limited. The signal can be further compounded by the presence of noise and PCR artifacts such as stutter which can mask or mimic biological alleles. Because manual interpretation methods cannot comprehensively account for such nuances, it would be v

SUBMITTER: Karkar S 

PROVIDER: S-EPMC6886162 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature