Intestinal microbiota domination under extreme selective pressures characterized by metagenomic read cloud sequencing and assembly.

ABSTRACT:

Background

Low diversity of the gut microbiome, often progressing to the point of intestinal domination by a single species, has been linked to poor outcomes in patients undergoing hematopoietic cell transplantation (HCT). Our ability to understand how certain organisms attain intestinal domination over others has been restricted in part by current metagenomic sequencing technologies that are typically unable to reconstruct complete genomes for individual organisms present within a sequenced microbial community. We recently developed a metagenomic read cloud sequencing and assembly approach that generates improved draft genomes for individual organisms compared to conventional short-read sequencing and assembly methods. Herein, we applied metagenomic read cloud sequencing to four s

SUBMITTER: Kang JB 

PROVIDER: S-EPMC6886166 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature