CRISPR-Cas13d mediates robust RNA virus interference in plants.

ABSTRACT:

Background

CRISPR-Cas systems endow bacterial and archaeal species with adaptive immunity mechanisms to fend off invading phages and foreign genetic elements. CRISPR-Cas9 has been harnessed to confer virus interference against DNA viruses in eukaryotes, including plants. In addition, CRISPR-Cas13 systems have been used to target RNA viruses and the transcriptome in mammalian and plant cells. Recently, CRISPR-Cas13a has been shown to confer modest interference against RNA viruses. Here, we characterized a set of different Cas13 variants to identify those with the most efficient, robust, and specific interference activities against RNA viruses in planta using Nicotiana benthamiana.

Results

Our data show that LwaCas13a, PspCas13b, and CasRx variants mediate high interference ac

SUBMITTER: Mahas A 

PROVIDER: S-EPMC6886189 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature