Aloin Preconditioning Attenuates Hepatic Ischemia/Reperfusion Injury via Inhibiting TLR4/MyD88/NF-κB Signal Pathway In Vivo and In Vitro.

ABSTRACT:

Background

Aloin exerts considerable protective effects in various disease models, and its effect on hepatic ischemia-reperfusion (HIR) injury remains unknown. This research is aimed at conducting an in-depth investigation of the antioxidant, anti-inflammatory, and antiapoptosis effects of aloin in HIR injury and explain the underlying molecular mechanisms.

Methods

In vivo, different concentrations of aloin were intraperitoneally injected 1 h before the establishment of the HIR model in male mice. The hepatic function, pathological status, oxidative stress, and inflammatory and apoptosis markers were measured. In vitro, aloin (AL, C₂₁H₂₂O₉) or lipopolysaccharide (LPS) was added to a culture of mouse primary hepatocytes before it

SUBMITTER: Du Y 

PROVIDER: S-EPMC6886335 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature