Genome-scale phylogenetics reveals a monophyletic Zoopagales (Zoopagomycota, Fungi).

ABSTRACT: Previous genome-scale phylogenetic analyses of Fungi have under sampled taxa from Zoopagales; this order contains many predacious or parasitic genera, and most have never been grown in pure culture. We sequenced the genomes of 4 zoopagalean taxa that are predators of amoebae, nematodes, or rotifers and the genome of one taxon that is a parasite of amoebae using single cell sequencing methods with whole genome amplification. Each genome was a metagenome, which was assembled and binned using multiple techniques to identify the target genomes. We inferred phylogenies with both super matrix and coalescent approaches using 192 conserved proteins mined from the target genomes and performed ancestral state reconstructions to determine the ancestral trophic lifestyle of the clade. Our results indi

SUBMITTER: Davis WJ 

PROVIDER: S-EPMC6886740 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature