Significant alteration of liver metabolites by AAV8.Urocortin 2 gene transfer in mice with insulin resistance.

ABSTRACT:

Introduction

Urocortin 2 (Ucn2) is a 38-amino acid peptide of the corticotropin-releasing factor family. Intravenous (IV) delivery of an adeno-associated virus vector serotype 8 encoding Ucn2 (AAV8.Ucn2) increases insulin sensitivity and glucose disposal in mice with insulin resistance.

Objective

To determine the effects of Ucn2 on liver metabolome.

Methods

Six-week-old C57BL6 mice were divided into normal chow (CHOW)-fed and high fat diet (HFD)-fed groups. The animals received saline, AAV8 encoding no gene (AAV8.Empt) or AAV8.Ucn2 (2x1013 genome copy/kg, IV injection). Livers were isolated from CHOW-fed and HFD-fed mice and analyzed by untargeted metabolomics. Group differences were statistically analyzed.

Results

In CHOW-fed mice, AAV8.Ucn2 gene transfer (vs.

SUBMITTER: Kim YC 

PROVIDER: S-EPMC6886859 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature