Single-cell RNA-seq reveals the invasive trajectory and molecular cascades underlying glioblastoma progression.

ABSTRACT: Glioblastoma (GBM) is the most common and aggressive primary brain tumor, in which GBM stem cells (GSCs) were identified to contribute to aggressive phenotypes and poor prognosis. Yet, how GSCs progress to invasive cells remains largely unexplored. Here, we revealed the cell subpopulations with distinct functional status and the existence of cells with high invasive potential within heterogeneous primary GBM tumors. We reconstructed a branched trajectory by pseudotemporal ordering of single tumor cells, in which the root showed GSC-like phenotype while the end displayed high invasive activity. Thus, we further determined a path along which GSCs gradually transformed to invasive cells, called the 'stem-to-invasion path'. Along this path, cells showed incremental expression of GBM invasion-a

SUBMITTER: Pang B 

PROVIDER: S-EPMC6887585 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature