CircHECTD1 mediates pulmonary fibroblast activation via HECTD1.

ABSTRACT:

Background

Circular RNA (circRNA), a new class of noncoding RNA, has been shown to be important in silicosis due to its unique role as a transcription regulator or as a sponge of small RNA regulators. Here, the mechanisms underlying circHECTD1/HECTD1 in fibroblast activation and subsequent fibrosis induced by SiO₂ were investigated.

Methods

Primary human pulmonary fibroblasts (HPF-a) were utilized, combined with quantitative real-time PCR (qRT-PCR) and fluorescence in situ hybridization (FISH) assays. LC3B-LV-RFP lentivirus was used to evaluate the role of autophagy. The CRISPR/Cas9 system was applied to specifically knock down HECTD1, combined with MTT, BrdU, and migration assays, to explore the functional changes induced by SiO₂.

Results

After

SUBMITTER: Chu H 

PROVIDER: S-EPMC6887829 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature