Macrophages reprogram after ischemic stroke and promote efferocytosis and inflammation resolution in the mouse brain.

ABSTRACT:

Aims

Blood-borne monocytes/macrophages infiltrate the brain in massive numbers after ischemic stroke, but their impact on poststroke brain injury and recovery remains elusive. This study examined the transcriptomic changes in monocytes/macrophages after ischemic stroke and the functional implications of these changes, particularly with regards to the contribution of these cells to the phagocytic clearance of dead/dying cells (efferocytosis) in the poststroke brain.

Methods

We performed whole-genome RNA sequencing on the monocyte/macrophage population sorted from mouse brain and peripheral blood 5 days after permanent focal cerebral ischemia. In addition, the spatial and temporal profiles of macrophage efferocytosis were examined in vivo by immunohistochemistry 3-7 days after

SUBMITTER: Zhang W 

PROVIDER: S-EPMC6887920 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature