Changes in Calcium Homeostasis and Gene Expression Implicated in Epilepsy in Hippocampi of Mice Overexpressing ORAI1.

ABSTRACT: Previously, we showed that the overexpression of ORAI1 calcium channel in neurons of murine brain led to spontaneous occurrence of seizure-like events in aged animals of transgenic line FVB/NJ-Tg(ORAI1)Ibd (Nencki Institute of Experimental Biology). We aimed to identify the mechanism that is responsible for this phenomenon. Using a modified Ca²⁺-addback assay in the CA1 region of acute hippocampal slices and FURA-2 acetomethyl ester (AM) Ca²⁺ indicator, we found that overexpression of ORAI1 in neurons led to altered Ca²⁺ response. Next, by RNA sequencing (RNAseq) we identified a set of genes, whose expression was changed in our transgenic animals. These data were validated using customized real-time PCR assays and digital droplet PCR (d

SUBMITTER: Majewski L 

PROVIDER: S-EPMC6888010 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature