Celiac Disease Monocytes Induce a Barrier Defect in Intestinal Epithelial Cells.

ABSTRACT: Intestinal epithelial barrier function in celiac disease (CeD) patients is altered. However, the mechanism underlying this effect is not fully understood. The aim of the current study was to evaluate the role of monocytes in eliciting the epithelial barrier defect in CeD. For this purpose, human monocytes were isolated from peripheral blood mononuclear cells (PBMCs) from active and inactive CeD patients and healthy controls. PBMCs were sorted for expression of CD14 and co-cultured with intestinal epithelial cells (IECs, Caco2BBe). Barrier function, as well as tight junctional alterations, were determined. Monocytes were characterized by profiling of cytokines and surface marker expression. Transepithelial resistance was found to be decreased only in IECs that had been exposed to celiac mon

SUBMITTER: Delbue D 

PROVIDER: S-EPMC6888450 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature