An integrated screening system for the selection of exemplary substrates for natural and engineered cytochrome P450s.

ABSTRACT: Information about substrate and product selectivity is critical for understanding the function of cytochrome P450 monooxygenases. In addition, comprehensive understanding of changes in substrate selectivity of P450 upon amino acid mutation would enable the design and creation of engineered P450s with desired selectivities. Therefore, systematic methods for obtaining such information are required. Herein, we developed an integrated P450 substrate screening system for the selection of "exemplary" substrates for a P450 of interest. The established screening system accurately selected the known exemplary substrates and also identified previously unknown exemplary substrates for microbial-derived P450s from a library containing sp³-rich synthetic small molecules. Synthetically potent

SUBMITTER: Kanoh N 

PROVIDER: S-EPMC6888865 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature