Tracing the first hematopoietic stem cell generation in human embryo by single-cell RNA sequencing.

ABSTRACT: Tracing the emergence of the first hematopoietic stem cells (HSCs) in human embryos, particularly the scarce and transient precursors thereof, is so far challenging, largely due to the technical limitations and the material rarity. Here, using single-cell RNA sequencing, we constructed the first genome-scale gene expression landscape covering the entire course of endothelial-to-HSC transition during human embryogenesis. The transcriptomically defined HSC-primed hemogenic endothelial cells (HECs) were captured at Carnegie stage (CS) 12-14 in an unbiased way, showing an unambiguous feature of arterial endothelial cells (ECs) with the up-regulation of RUNX1, MYB and ANGPT1. Importantly, subcategorizing CD34⁺CD45^- ECs into a CD44⁺ population strikingly enriched

SUBMITTER: Zeng Y 

PROVIDER: S-EPMC6888893 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature