Protection induced by malaria virus-like particles containing codon-optimized AMA-1 of Plasmodium berghei.

ABSTRACT:

Background

Despite the extensive endeavours, developing an effective malaria vaccine remains as a great challenge. Apical membrane antigen 1 (AMA-1) located on the merozoite surface of parasites belonging to the genus Plasmodium is involved in red blood cell invasion.

Methods

Influenza virus-like particle (VLP) vaccines containing codon-optimized or native (non-codon optimized) AMA-1 from Plasmodium berghei were generated. VLP-induced protective immunity was evaluated in a mouse model.

Results

Mice immunized with VLP vaccine containing the codon-optimized AMA-1 elicited higher levels of P. berghei-specific IgG and IgG2a antibody responses compared to VLPs containing non-codon optimized AMA-1 before and after challenge infection. Codon-optimized AMA-1 VLP vaccination i

SUBMITTER: Lee DH 

PROVIDER: S-EPMC6888966 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature